shiga toxin-producing escherichia coli isolated from lettuce samples in tehran, iran

نویسندگان

somayeh mazaheri department of biology, alzahra university, tehran, ir iran; department of biology, alzahra university, tehran, ir iran. tel: +91-98012807

siavosh salmanzadeh ahrabi department of biology, alzahra university, tehran, ir iran

mohammad mahdi aslani department of microbiology, pasteur institute of iran, tehran, ir iran

چکیده

conclusions this study indicated the contamination of lettuce by stec strains and its possible role as the source of infection. resistance to both tetracycline and ampicillin may be considered as an emergency alarm for a multidrug resistance of stec strains. results eight samples were positive for presence of stec strains, three contained stx1, five contained stx2, and one sample was positive for presence of both rbfo157 and flich7. they were susceptible to all the antibiotics except for ampicillin and tetracycline. objectives the aim of this study was to evaluate the prevalence and antibiotic resistance pattern of stec strains in lettuce samples. since lettuce is used as a raw vegetable in salads, the rates of infections caused by this vegetable are high. materials and methods a total of 100 samples collected from tehran, iran, were transported to the laboratory, homogenized by a stomacher in e. coli broth containing cefixime, and cultured on macconkey agar medium. their dna was extracted by boiling method and polymerase chain reaction (pcr) was performed, using five primers targeting the stx1, stx2, flich7, rbfo157, and eaea genes. susceptibility testing against ampicillin, imipenem, cephalosporin, tetracycline, aminoglycosides, chloramphenicol and quinolones was performed using disk diffusion method. background during the last decade, the prevalence of foodborne diseases due to contaminated food as well as the outbreaks of diseases due to shiga toxin-producing escherichia coli (stec) strains has increased.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Shiga Toxin-Producing Escherichia Coli Isolated From Lettuce Samples in Tehran, Iran

BACKGROUND During the last decade, the prevalence of foodborne diseases due to contaminated food as well as the outbreaks of diseases due to Shiga toxin-producing Escherichia coli (STEC) strains has increased. OBJECTIVES The aim of this study was to evaluate the prevalence and antibiotic resistance pattern of STEC strains in lettuce samples. Since lettuce is used as a raw vegetable in salads,...

متن کامل

Prevalence, Molecular Characterization and Serology of Shiga toxin-Producing Escherichia coli Isolated from Buffaloes in West Azerbaijan, Iran

This present study is the first to report the presence of Shiga toxin-producing Escherichia coli (STEC) in buffaloes in Iran. A total of 360 fecal samples were collected from buffaloes from different regions in the west Azerbaijan province of Iran and cultured for the isolation of E. coli using routine biochemical tests. From the fecal samples, 340 E. coli were isolated and, of these, 26 STEC i...

متن کامل

Detection of Shiga toxin-producing Escherichia coli (STEC) in faeces of healthy calves in Mashhad, Iran

The aim of this study was to identify virulent Shiga toxin-producing Escherichia coli (STEC) strains isolated from faecal samples of 100 clinically healthy calves. In the present study, a total of 100 Escherichia coli (E. coli) isolates from clinically healthy calves belonging to 6 different farms located in Khorasan Razavi province, Iran, were examined for presence of virulence genes character...

متن کامل

Shiga toxin-producing Escherichia coli

Shiga toxin-producing Escherichia coli (STEC) cause hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS) in humans. Outbreaks are linked to bovine food sources. STEC O157:H7 has been responsible for the most severe outbreaks worldwide. However, non-O157 serotypes have emerged as important enteric pathogens in several countries. The main virulence factor of STEC is the production of Shig...

متن کامل

Shiga Toxin – producing Escherichia coli

1. Rautio M, Lonnroth M, Saxen H, Nikku R, Väisanen ML, Finegold SM, et al. Characteristics of an unusual anaerobic pigmented gram-negative rod isolated from normal and infl amed appendices. Clin Infect Dis. 1997;25(Suppl 2):S107–10. 2. Rautio M, Saxen H, Siitonen A, Nikku R, Jousimies-Somer H. Bacteriology of histopathologically defi ned appendicitis in children. Pediatr Infect Dis J. 2000;19:...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
jundishapur journal of microbiology

جلد ۷، شماره ۱۱، صفحات ۰-۰

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023